Hepatoprotective and Hypoglycemic activities  of  Morinda tinctoria leaves extract on  alloxan induced diabetic albino rats.

V.Ramasubramanian; K.Palanivelu

Volume : VI, Issue : IV, April - 2016

Hepatoprotective and Hypoglycemic activities of Morinda tinctoria leaves extract on alloxan induced diabetic albino rats.

V. Ramasubramanian, K. Palanivelu

Abstract :

The present study explores the hepatoprotective and hypoglcemic effect of Morinda tinctoria in alloxan induced diabetic rats. Alloxan (120mg/kg body weight of rats) induces chemical diabetes in a wide variety of animal species through damage of insulin secreting cell. The M. tinctoria leaf extract was prepared with ethanol and ethyl acetate solvents. Preliminary phytochemical screening of phenols, flavonoids, alkaloids, terpenoids and tannins were performed by standard protocols. Compounds responsible for these activities were identified by GC–MS with the inbuilt liaries. After 35 days of oral administration of M. tinctoria ethanol (250 mg/kg b.w) and M. tinctoria ethyl acetate (200 mg/kg b.w) extracts treatment, the physical and biochemical parameter such as body weight, blood glucose, lipid profile, hepatic markers and antioxidant parameters are assayed. Hypoglycemic activity may due to its stimulatory effect at cellular level on islets. Ethyl acetate extract of M. tinctoria produced more significant reduction in glucose, HbA1C, lipid profile, and enhanced hepatic marker enzymes and antioxidant activity in alloxan induced diabetic rats. Ethyl acetate extract yielded more promising results than the ethanol extract and even more or less same with the drug glibenclamide. The preliminary investigation on the hepatoprotective and hypoglycemic efficacy of ethyl acetate extract of M. tinctoria exhibit significant role due to its high alkaloid, terpenoid, coumarin and polyphenol contents. Further studies will be carried out to elucidate its active fraction and its mechanism of action.