HISTOPATHOLOGICAL STUDY ON CYTOPROTECTIVE EFFECT OF LYCOPENE ON ALLOXAN INDUCED DIABETES IN RATS

Dr.V.AKILANDESWARI; Dr.S.VASANTH

Volume : VII, Issue : XI, November - 2017

HISTOPATHOLOGICAL STUDY ON CYTOPROTECTIVE EFFECT OF LYCOPENE ON ALLOXAN INDUCED DIABETES IN RATS

Dr. V. Akilandeswari, Dr. S. Vasanth

Abstract :

DM virtually affects every system of the body mainly due to metabolic disturbances caused by hyperglycemia and associated with abnormalities in carbohydrate, fat and protein metabolism and results in chronic complicationsincluding microvascular, macrovascular and neuropathic disorders. we planned a study to evaluate the the cytoprotective effect of lycopene in alloxan induced diabetes in rats by doing histopathological examination of pancreas and kidney. The rat was divided in to 5 groups of six each(n=6). GROUP I (n=6) Normal control.GROUP II (n=6) Diabetic rats GROUP III (n=6) Diabetic rats treated with protamine zinc insulin 0.9 U per 100 gm s.c along with normal diet.GROUP IV (n=6) Treated with Lycopene 2.5 mg/kg orally 2weeks before and 3weeks after induction of diabetes mellitus. GROUP V (n=6) Treated with Lycopene 5mg/kg orally 2 weeks before and3 weeks after induction of diabetic rats. Lycopene at 2.5mg/kg given before and after induction of diabetes showed significant protective effect. Rats treated with 5mg/kg of Lycopene showed regenerating beta cells in the islets of Langerhans in the pancreatic section. The pancreatic section of rats treated with Protamine zinc insulin also showed vacuolated beta cells and moderate infilteration of inflammatory cells in the Islets of Langerhans.. Diabetic Rats treated with Lycopene 2.5mg/kg showed renal tubules with less vacuolation and mild intertubular hemorrhages. But at 5mg/kg showed normal architecture of renal tubules and glomerulus. In diabetic rats treated with Protamine zinc insulin showed , mild vacuolation of renal tubules and normal glomerulus.