Volume : VII, Issue : XII, December - 2017

HIV RNA viral load by Real Time PCR: A preliminary investigation form infected population.

Dr. T. Santhi, Dr. G. Kiran Kumar

Abstract :

 Acquired immunodeficiency Syndrome (AIDS) is  the most prevalent disease all over the world. It is caused by an infective retrovirus HIV (Human Immunodeficiency Virus). It was first isolated from South Africa. HIV disease also characterized by a gradual deterioration of an individual’s defense system as infection spreads. This may lead to the final stage termed “acquired immunodeficiency syndrome” (AIDS). “Syndrome” is used to describe the cluster of medical conditions that can batter an HIV–infected person’s immune system. Crucial immune cells called CD4 cells (also known as T–helper cells) are disabled and killed during the typical course of infection. The dynamic range of Real–time RT–PCR assay makes it particularly useful for quantifying full range of virus load observed in treated and untreated patients with HIV infection. In our study out of 37 samples were collected from HIV infected population who were attended to department of DVL and Surgery department, KGH, Visakhapatnam between Feuary 2016 to January 2017 for the regular check up. Out of 37 samples, 32 were showed standard curve means positive results where as CD4 count shows <200/mL. Results revealed that the Ct values are ranging from 25.39– 34.87. The dynamic range of Real–time RT–PCR assay makes it particularly useful for quantifying full range of virus load observed in treated and untreated patients with HIV infection.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Dr. T. Santhi, Dr. G. Kiran Kumar, HIV RNA viral load by Real Time PCR: A preliminary investigation form infected population., INDIAN JOURNAL OF APPLIED RESEARCH : Volume-7 | Issue-12 | December-2017

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