Human Adenovirus in hospitalised children at a tertiary care center in Jaipur

M Anjaneya Swamy; Bharti Malhotra; P V Janardhan Reddy; Neeraj Kumar; Jitendra Kumar Tiwari

Volume : V, Issue : X, October - 2015

Human Adenovirus in hospitalised children at a tertiary care center in Jaipur

M Anjaneya Swamy, Bharti Malhotra, P V Janardhan Reddy, Neeraj Kumar, Jitendra Kumar Tiwari

Abstract :

 Background: Human Adenovirus (HAdV) causes Acute Respiratory Infections (ARIs) with significant mortality and morbidity in infants and Children. Aim: To determine the occurrence of HAdV in hospitalised children of age ≤ 5 years. Materials and Methods: Sterile viral transport media was used for the collection of throat swabs and nasopharyngeal aspirates from children with ARI. Viral nucleic acid was extracted by using automated nucleic acid extractor (Biomeuriex). Real time RT–PCR was done by using HAdV specific primer probe on ABI 7500 Fast Dx (Life Technologies, USA) Results: The total positivity for HAdV was found to be 25/225 (11.11%). Among the total positive samples males were predominantly infected with HAdV in 20/25(80.0%) than females in 5/25(20.0%). Human Adenovirus was predominant in the age group of 1–12 months (84.0%). HAdV positive patients presented with cough in 25/25(100.0%), fever in 24/25(96.0%), and pneumonia in 8/25(32.0%). Highest peak of HAdV was observed in the month of Feuary 2014. Conclusion: HAdV was associated with 11.11% of hospitalised children with ARI. 32.0% of HAdV positive patients had pneumonia. Adenovirus should be tested in children with ARI. Unnecessary use of antibiotics can be prevented by timely detection of HAdV.