Human bile impairs the viability of hepatocellular carcinoma cells in vitro.

Renato Ferreira da Silva; Jucimara Colombo; Livia Carvalho Ferreira; Thaiz Ferraz Borin; Ilka de Fatima Santana Ferreira Boin; Vitor Miranda Fonseca Santos; Rita de Cassia Martins Alves da Silva; Debora Aparecida Pires de Campos Zuccari

Volume : VIII, Issue : I, January - 2018

Human bile impairs the viability of hepatocellular carcinoma cells in vitro.

Renato Ferreira Da Silva, Jucimara Colombo, Livia Carvalho Ferreira, Thaiz Ferraz Borin, Ilka De Fatima Santana Ferreira Boin, Vitor Miranda Fonseca Santos, Rita De Cassia Martins Alves Da Silva, Debora Aparecida Pires De Campos Zuccari

Abstract :

Background/aims: Hepatocellular carcinoma is the fifth most common variety of cancer. In over 50% of cases it will spread to the blood vessels, reducing the range of curative treatment options. While vascular invasions occur frequently, biliary invasion is rare and also suggest poor prognosis. This study assesses factors exist in the biliary tree or in the bile capable of preventing cancer cell proliferation. Thus, this experimental in–vitro study was designed to analyze the viability of HCC cells after treatment with human bile. Methods: HepG2 cells were cultured in Dulbecco‘s modified Eagle‘s medium and were divided into 5 groups: one untreated control and four indicated for treatment with different concentrations of human bile (6.25%, 12.5%, 25% and 50%). The Research Ethics Committe of the São José do Rio Preto Medical School approved this protocol (number 830.862). Results were analyzed using the ANOVA and Bonferroni tests. Results: In three of the four treated groups there was a significant decrease in cell viability compared with the control group (P<0.05) and there was a dose–dependent reduction in cell viability (P<0.05). Conclusion: Human bile has the ability to diminish the viability of human HCC cells.