HUMAN PAPILLOMAVIRUS DETECTION IN THE WOMEN OF JAMMU REGION OF JAMMU AND KASHMIR: A HOSPITAL BASED STUDY.

Rohit Bhardwaj; Meenakshi Bhagat; Rakesh Kumar Panjaliya; Kuldeep Kumar Sharma; Parvinder Kumar

Volume : V, Issue : VII, July - 2015

HUMAN PAPILLOMAVIRUS DETECTION IN THE WOMEN OF JAMMU REGION OF JAMMU AND KASHMIR: A HOSPITAL BASED STUDY.

Rohit Bhardwaj, Meenakshi Bhagat, Rakesh Kumar Panjaliya, Kuldeep Kumar Sharma, Parvinder Kumar

Abstract :

&nbsp;Background: Cervical cancer is most common malignancy in females in the age group of 25&ndash;65 years and is also the commonest cause of female death. Human papillomavirus (HPV) has been identified as the significant risk factor for this cancer. Therefore, the present study is designed to check the prevalence of HPV and HR&ndash;HPV 16, 18, 31 &amp; 33 infections in the women of Jammu region of J&amp;K state. Methods: Cervical tissue samples (exfoliates) of 500 women, attending OPD of different hospitals of Jammu region with varied complaints, were collected and were screened for the presence of HPV genome by using polymerase chain reactions (PCR). Samples found positive for HPV were further analyzed for pinpointing the specific type of high risk HPV therein by using type specific primers for HPV 16, 18, 31 &amp; 33. Results: Out of 500 samples 40.8% were found to be positive for HPV DNA. Among them high risk HPV&ndash;16 and HPV&ndash; 18 was the most common and observed in 9.2% samples followed by HPV&ndash;33 and HPV&ndash;31 (1% and 0.8% respectively). Higher HPV percentage was observed in women with &gt;65 years of age (71.53%), married before 18 years of age (48.44%) and with 4 or more children (50%). The study is first of its kind in Jammu region and the results have been compared with the available data on North Indian females reported by various workers. Conclusions: The study shows high prevalence of high&ndash;risk HPV DNA in cervical tissue of women of the Jammu region of J&amp;K State. The data generated will be useful for laying the future guidelines for molecular genetic screening and treatment of HPV infected women in the state.