Identification and in�silico analysis of a small heat shock protein (HSP26) expressed in the Bombyx mori due to heat shock

Punyavathi; H. B. Manjunatha

Volume : VII, Issue : I, January - 2017

Identification and in�silico analysis of a small heat shock protein (HSP26) expressed in the Bombyx mori due to heat shock

Punyavathi, H. B. Manjunatha

Abstract :

Protein extracted from different instars male and female larvae of Bombyx mori exposed to heat shock at 40°C was subjected for identification and in–silico analysis. Heat shock induced protein bands with molecular mass of 25 kDa excised from the 1–DE gel of male and female larvae was analyzed by MALDI–TOF–MS. The resultant peptide mass fingerprints denoted it as small heat shock protein 26 kDa and found localized on the chromosome 27 of B. mori. The multiple sequence alignment and phylogenetic tree clearly indicates that BmHSP26 is species–specific. The Epiloc program while determine the location of BmHSP26 as cytoplasmic, the physico–chemical properties of BmHSP26 with 80.81 aliphatic index and disulphide idge between Cys78 and Cys85 indicate its thermo–stability in association with two major HSPs – Hsp90 and Hsc70–4. Accordingly, we suppose that BmHSP26 along with other HSPs help the silkworm larvae acquire tolerance to overcome thermal stress.