Identification of Nitrogen fixing Cyanobacteria by PCR amplification of nif genes

Rahul Anand; Bhavana Singh; Divya Kumari; Deepak Kumar

Volume : VI, Issue : VIII, August - 2016

Identification of Nitrogen fixing Cyanobacteria by PCR amplification of nif genes

Rahul Anand, Bhavana Singh, Divya Kumari, Deepak Kumar

Abstract :

Nitrogen fixation is a common phenomenon exhibited by many organisms to fix environmental source of Nitrogen into simple utilisable form. Among various such organisms, Cyanobacteria possess nif genes, which can be identified at molecular level by Polymerase Chain Reaction. Cyanobacteria can be used as a potential Bio–Fertiliser for Kharif crops such as rice, maize, soybean, groundnuts, etc. The current study focuses on identifying nif genes containing Cyanobacteria from Ranchi, Jharkhand. The conserved sequences present on nif genes provide sufficient sequence information to construct primers, which were used to amplify nif genes.