Volume : IV, Issue : VI, June - 2014

Identification of Rynaxypyr resistant Plutella xylostella with RAPD–PCR

Navid N Patel, Dr. K N Patel, Vina Sagane

Abstract :

: RAPD PCR analysis was performed to know the molecular mechanism of rynaxypyr resistance in P. xylostella. Diamond backmoth(DBM) was selected against rynaxypyr upto seven generations. Selection process resulted in about 34.88 folds resistance to that of homogeneous susceptible F5 generation. RAPD–PCR analysis of rynaxypyr resistant and susceptible strain of P. xylostella showed considerable difference. Out of 20 primers screened 17 primers showed the amplification in which 11 primers found to be polymorphic in nature. Two primers gave maximum polymorphism and found potential markers for rynaxypyr resistance in DBM. Analysis of amplicons indicated that rynaxypyr treatment produced distinct genetic alterations in surviving P. xylostella.  

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Navid N Patel, Dr. K N Patel, Vina Sagane Identification of Rynaxypyr resistant Plutella xylostella with RAPD-PCR Indian Journal of Applied Research, Vol.IV, Issue.VI June 2014

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