Imbalance between peroxisomal ROS generating enzymes and ROS scavenging enzymes may contribute to cyclophosphamide induced renal damage in rats

Premila Abraham; Bina Isaac

Volume : VI, Issue : XI, November - 2016

Imbalance between peroxisomal ROS generating enzymes and ROS scavenging enzymes may contribute to cyclophosphamide induced renal damage in rats

Premila Abraham, Bina Isaac

Abstract :

The mechanism of renal toxicity of cyclophosphamide (CP), a commonly used anticancer drug is not clear. In a recent study we have demonstrated increase in size and number of peroxisomes in the kidneys of rats after treatment with cyclophosphamide. Our aim is to examine whether there is any alterations in the expressions of peroxisomal ROS metabolising enzymes in the kidneys of rats upon CP treatment. Rats received single i.p. injection of 150 mg/kg body wt. and killed 24 hrs.later. The protein expression and activities of ROS generating enzymes (xanthine oxidase, fatty acid oxidase, D amino acid oxidase, polyamine oxidase, and urate oxidase) and activities ROS scavenging enzymes (catalase, glutathione peroxidase and glutathione transferase) were determined in the kidney peroxisomal fractions by western blot and spectrophotometric methods respectively. A significant increase in the protein levels of the ROS generating enzymes (except urate oxidase) was observed in the peroxisomal fractions of CP treated rat kidneys. The activity of catalase was increased, while the activities of other antioxidant enzymes remained unaltered upon CP treatment. It is concluded that peroxisomal dysfunction i.e. imbalance between the ROS generating enzymes and ROS scavenging enzymes may contribute to CP induced renal damage