IMMUNOHISTOCHEMICAL PROFILE OF HIGH RISK HUMAN PAPILLOMA VIRUS IN PRECANCEROUS LESIONS OF CERVIX

Dr.Sudha.V; Dr.Lalitha Rani.N

Volume : VII, Issue : VI, June - 2017

IMMUNOHISTOCHEMICAL PROFILE OF HIGH RISK HUMAN PAPILLOMA VIRUS IN PRECANCEROUS LESIONS OF CERVIX

Dr. Sudha. V, Dr. Lalitha Rani. N

Abstract :

INTRODUCTION: Carcinoma of the cervix is the second most common neoplasm in women world wide. Cervical cancer is related to the human papilloma virus. Viral E6 and E7 proteins are responsible for oncogenic effects of HPV. They promote DNA synthesis in viral affected cells and increase expression of P16. E6 oncogene inhibits apoptosis, inhibits P53 and increases the expression of Ki67 in injured cells. AIM: To assess the potential use of immunohistochemical markers such as HPV (Panmarker), P16, and Ki67 in grading of intraepithelial lesions and to detect the risk of development of cervical cancer. Materials and methods: 30 cervix biopsies with intraepithelial lesion were selected and submitted to immunohistochemical study for biomarker HPV (Panmarker), P16 and Ki67. Results: 33% of CIN I lesions, 22% of CIN II lesions and 11% of CIN III lesions show positivity for HPV panmarker. 50% of CIN I cases and 66.6% of CIN II cases expressed P16. 90% of CIN III cases expressed P16. There was higher expression of Ki 67 in CIN III (66%) lesions than CIN II(44%) and CIN I (16%). P16 marker score was higher in CIN III lesions. CONCLUSIONS: P16 is a potential biomarker used for the diagnosis of dysplasia, tumor progression and associated with high risk HPV oncogenes. Coexpression of p16 and Ki67 could help in grading of intraepithelial lesions and to detect the risk of developing cervical carcinoma.