Immunological Study about the Efficacy of Various Antidiabetic Drugs on a Combination of High Fat Diet Fed and Low Dose of Streptozotocin Treated Rats as a Model for Type 2 Diabetes

Marwa A. El-Beltagy; Sherif Y. Saleh; Abeir A. Shalaby; Abd El-Rehim A. El-Ghannam; Ibrahim A. Ibrahim

Volume : V, Issue : VI, June - 2015

Immunological Study about the Efficacy of Various Antidiabetic Drugs on a Combination of High Fat Diet Fed and Low Dose of Streptozotocin Treated Rats as a Model for Type 2 Diabetes

Marwa A. El 45 Beltagy, Sherif Y. Saleh, Abeir A. Shalaby, Abd El 45 Rehim A. El 45 Ghannam, Ibrahim A. Ibrahim

Abstract :

The present study was aimed to investigate the action of various antidiabetic drugs in improvement insulin resistance and level of pro–inflammatory cytokines (TNF–α, IL–6, CRP), which can contribute in diabetic vascular complications in rats fed HFD and low dose of Streptozotocin as a model for Type II Diabetes (T2DM). Ninety Sprague–Dawley (SD) male rats were included in this study, from which 15 were randomly selected as the normal control group (group A) and the remaining 75 were considered as T2DM model group. Group A was fed with commercial balanced diets and the T2DM model group, with high fat diets ((58% fat, 25% protein and 17% carbohydrate, as a percentage of total kcal). After 6–week feeding, the T2DM was injected i.p. with low dose of streptozotocin (STZ) (25 mg kg−1)then randomly allocated into four groups according to the drug treatment, Diabetic (group B), Janumet (group C), janumet plus pioglitazone (group D), and metformin plus pioglitazone group (group E). After 4 and 8 weeks from beginning of treatment, body weight, fasting blood glucose levels, fasting insulin level and HbA1c were measured. Serum levels of pro–inflammatory cytokines (TNF–α, IL–6, hs–CRP), IgM and IgG were determined using the enzyme–linked immunosorbent assay (ELISA).Also serum creatinine, urea and SGPT were estimated. All data were statistically analyzed. Diabetes caused a significant increase in blood levels of glucose, insulin, HbA1c, TNF–α, IL–6, Compared with treated groups which show a decrease in blood levels of glucose, HbA1c , levels of pro–inflammatory cytokines (TNF–α, IL–6, CRP) also lowered, all this improve insulin sensitivity in this groups beside serum creatinine, urea and SGPT level in all groups not significant affected. This study indicated that antidiabetic drugs used were improved carbohydrates metabolism, insulin sensitivity and pro–inflammatory cytokines if compared with diabetic groups beside has no damage effect on kidney and liver tissue