In vitro fertilization related with mutation of mitochondrial COX1 gene in sheep

Saad Akram Hatif; Hayder Abdul–KareemAL–Mutar

Volume : VI, Issue : III, March - 2016

In vitro fertilization related with mutation of mitochondrial COX1 gene in sheep

Saad Akram Hatif, Hayder Abdul Kareemal Mutar

Abstract :

This study was conducted to investigate the possibility of occurrence genetic mutations in the emyos by in vitro fertilization of oocyte in sheep. The recovery of 450 oocytes from follicles in abattoir , it was incubated in the CO2 incubator , the oocytes maturation 320 .When In vitro fertilization by adult ram semen and follow up cell division of emyos to 2cell stage ,4cells stage and 8cells stage , the number of emyos 30 ,7 ,and 2 respectively . Genomic DNA extraction from 2 cell stages ,4cell stages ,and 8cell stages , followed by PCR to cytochrome oxidase subunit 1 (CXO1) gene , The sequencing of ovis CXO1 gene amplified product from 8cells, Sequence ID: gb|KC669585.1|, shows 99%, compatibility, score 1116 compare with Ovis aries eed Naemi5 (CXO1) gene from 2 cell,and emb|HE577849.1| 93% compatibility ,score 881 compare with Ovis aries, Assaf eed COX1gene .While a 4cells sequence ID Iemb|HE577849.1| show 93% compatibility score 880 compare with Ovis aries, Assaf eed COX1 from 2 cell. However, the polymorphism of COX1 genes of 8 cell after fertilizing was found to be compatibly 93% the result appeared conformation of protein CO1 for two , four and eight cells , the lack of similarity of conformation of protein ,the difference in the order of the amino acids and the emergence of site side of acids as in shape and the difference in body protein leads to variation in the function of CXO1gene In conclusion there was variation of mutation in two cells stage compared with four cells and eight cells stage in vitro fertilization of COX1 gene .This study is the first according to the information in Iraq Awassi sheep which were registered as a genius of Awassi sheep in Iraq in NCBI.