Volume : IV, Issue : X, October - 2014

In vitro plantlet regeneration from Hypocotyl and Root explants of Murraya koenigii (L.) Spreng

Nisha Khatik, Ramesh Joshi

Abstract :

Protocol for high frequency plantlet regeneration from Hypocotyl and Root explants from in vitro raised seedlings of Murraya koenigii was developed. The surface sterilized seeds were inoculated on half strength Murashige and Skoog (MS) basal medium for germination. Explants were obtained from 7–8 weeks old axenic seedlings & cultured on modified MS medium supplemented with 6–benzylaminopurine (BAP) 13.05 µM, Kinetin (Kin) 9.15 µM and Adenine sulphate (ADS) 148.45 µM alone or in combination to induce in vitro multiple shoots. The best result observed for the average number of shoots (94.5 ± 0.05%) was from root segment explants, which was significantly higher than hypocotyls (74.0 ± 0.04%). The elongated shoots were shifted to MS basal medium supplemented with indole–3–butyric acid (IBA) 19.72 µM for root induction. Plantlets were acclimatized and established in soil where they exhibited normal growth.

Keywords :

Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Nisha Khatik,Ramesh Joshi In vitro plantlet regeneration from Hypocotyl and Root explants of Murraya koenigii (L.) Spreng. Indian Journal of Applied Research, Vol.4, Issue : 10 October 2014

Number of Downloads : 725

References :