In Vitro Sterilization Protocol for Micropropagation  of Musa (AAA group) �Amritsagar� Musa (AAB group)  �Malbhog� and Musa (AAB group) �Chenichampa�  Banana

N K Goswami; Dr. P. J. Handique

Volume : III, Issue : VI, June - 2013

In Vitro Sterilization Protocol for Micropropagation of Musa (AAA group) �Amritsagar� Musa (AAB group) �Malbhog� and Musa (AAB group) �Chenichampa� Banana

N K Goswami, Dr. P. J. Handique

Abstract :

Sterilization procedure was standardized for three popular banana cultivars viz., Musa (AAA group) ‘Amritsagar’; Musa (AAB group) ‘Malbhog’; and Musa (AAB group) ‘Chenichampa’. Two sterilants, sodium hypochlorite (0.5 % and 1.0 %) and mercuric chloride (0.1 %) were used both singly and in combination for surface sterilization of explants under different exposure time ranging from 5 to 15 minutes. Sterilized explants were inoculated on four different MS media viz., MS Basal, MS+BAP 0.2 mg L–1, MS +BAP 0.3 mg L–1 and MS +BAP 0.5 mg L–1 to evaluate the response of different chemicals. The observations were recorded regularly up to 30 days with respect to the dead cultures, infected cultures and healthy cultures. Result showed that a treatment combination of Sodium hypochlorite (1.0%) for 15 minutes followed by HgCl2 (0.1%) for 7 minutes gives the highest percentage of aseptic culture establishment in in vitro condition.