In-vitro study of povidone-iodine and gentamycin on biofilm strains of In-situ urinary-catheter to determine their combined efficacy to prevent

Dr. Sudipta Kumar Maity; Dr. Purnima Mondal; Prof.(Dr.) Prasanta Kumar Maity

Volume : X, Issue : VII, July - 2020

In-vitro study of povidone-iodine and gentamycin on biofilm strains of In-situ urinary-catheter to determine their combined efficacy to prevent

Dr. Sudipta Kumar Maity, Dr. Purnima Mondal, Prof. Dr. Prasanta Kumar Maity

Abstract :

he biocides are generally used for perineal cleaning prior to urinary tract catheterisation and to luicate the passage of urinary catheter. These are also included in the urine drainage bags every time they are emptied. The catheter–meatal junction is also cleaned to minimise the periurethral uropathogens. Systemic prophylaxis with suitable antibiotic is also recommended to prevent catheter–associated UTI when indwelling catheters are used. In spite of implementation of this comprehensive policy in patients with long–term indwelling catheter, catheter–associated urinary tract infection (CAUTI) is the usual outcome. In a long–term(>30 days) catheterised patient with continuous drainage, the iatrogenic or ascending infections may not revealed as significant bacteriuria; rather they colonise on indewelling urinary catheter in the form of biofilm & serve as persistent source of treatment refractory infections by time to time dispersion of biofilm fragments. In this study, the biofilm mode of growth on long–term (30 d) indwelling uro–catheters have been studied, sensitivity of biofilm isolates were tested to povidone–iodine (urinary antiseptic) & gentamycin( antibiotic used for UTI prophylaxix) . Analysis of antibiotic resistance pattern and the antiseptic sensitivity of the biofilm strains to povidone–iodine were analysed to decide ,wheather there is a co–relation between antibiotic &biocide resistance, also regarding association of resistant genes. But it is clear that, long–term extensive use of povidone–iodine in catheterised urinary tract, where it has to deal with a rich and varied bacterial flora; was counter–productive and led to infections by drug–resistant Nosocomial pathogens. The study was conducted at IPGME & R, Kolkata in 2011.