Initiation of suspension culture and somatic  embryogenesis to plant regeneration in multipurpose  bamboo (Dendrocalamus tulda)

C. M. Wadatkar; N.V. More; V.V. Patil

Volume : IV, Issue : I, January - 2014

Initiation of suspension culture and somatic embryogenesis to plant regeneration in multipurpose bamboo (Dendrocalamus tulda)

C. M. Wadatkar, N. V. More, V. V. Patil

Abstract :

The cell aggregates disperse in liquid medium under orbital shaker is known as suspension culture. The suspension culture of Dendrocalamus tulda was performed by using callus. The friable callus tease aseptically and transferred in liquid medium .The culture obtained with clumps of proliferating globular, heart stages emyo with very little non emyogenic tissue. The number and size of somatic emyo clumps was measured to quantify the growth of emyo. The suspension culture were subculture every 21 d by replacing the old medium with equal volume of fresh medium. For the establishment of callus culture and suspension culture initiation various exogenous combinations of auxins and cytokinin (2,4-D, BAP,NAA) was found to be most effective. The somatic emyos (Globular, heart stages) were cultured on MS medium containing BAP and 2, 4-D were used for shoots regeneration .The shooted plants were subculture on rooting medium. The regenerated plants were hardened. The regenerated plants were found to be phonotypicaly normal. The harden plants were ready for field trails.