ISOLATION AND CHARACTERIZATION OF ENTEROCOCCUS FROM VARIOUS CLINICAL SAMPLES IN TERTIARY CARE INSTUTE WITH SPECIAL REFERENCE TO VRE (VANCOMYCIN RESISTANT ENTEROCOCCUS )AND VISE (VANCOMYCIN INTERMEDIATE SENSITIVE ENTEROCOCCUS)

Gajendra Singh Parihar; VaibhavMisra; Shashi Gandhi; Pramod Kushwaha

Volume : VIII, Issue : IV, April - 2018

ISOLATION AND CHARACTERIZATION OF ENTEROCOCCUS FROM VARIOUS CLINICAL SAMPLES IN TERTIARY CARE INSTUTE WITH SPECIAL REFERENCE TO VRE (VANCOMYCIN RESISTANT ENTEROCOCCUS )AND VISE (VANCOMYCIN INTERMEDIATE SENSITIVE ENTEROCOCCUS)

Gajendra Singh Parihar, Vaibhavmisra, Shashi Gandhi, Pramod Kushwaha

Abstract :

BACKGROUND: Enterococcus, an indigenous flora of the intestinal tract, is known to be relatively avirulent in healthy individuals, but turns as pathogens in hospitalized patients and are emerging nosocomial pathogen. OBJECTIVE: To Isolate and characterise Enterococci and to determine their antibiotic resistance pattern with special reference to VRE and VISE.MATERIAL AND METHODS: This prospective study was conducted in the Department of Microbiology,Gajra Raja Medical College,Gwalior. Isolation and Identification was done by conventional methods and antibiotic susceptibility testing was done by Kirby–Bauer Disc Diffusion method andMIC was determined by an E test strip.RESULT: In this study enterococci were mostly isolated from urine 65 (59.09%), followed by blood 26 (23.63%) and pus 14 (12.72%). Seven isolate of E. faecalis (7.60%) was found resistant to vancomycin by Kirby–Bauer Disc Diffusion test and resistance was of high level (MIC>256 mgm/dl). Vancomycin resistance was detected in E. faecium 9 (50%). CONCLUSION: Only two enterococal species were isolated in this study. They were E. faecalis92 (83.63%) and E. faecium18 (16.36%). In E. faecalis, 84 (91.30%) isolates showed resistance to penicillin. In E. faecalis, 58 (63.04%) isolates showed resistance to ampicillin . In E. faecalis, 50(54.34%) isolates showed Gentamycin Resistance. In E. faecalis, 51(55.43%) isolates showed Streptomycin Resistance. Seven vancomycin resistant E. faecalis (VRE) was isolated in this study. These isolate was highly resistant to vancomycin showing MIC > 256 mgm/dl.All the strain of enterococcus isolates were sensitive to linezolid.