Isolation and Characterization of Kocuria Rosea from Paper Industry Waste and Production of Cellulase Enzyme

Mr. Kalpesh Vishe; Ms. Divya More; Dr. Suman K. Satyarum

Volume : X, Issue : V, May - 2020

Isolation and Characterization of Kocuria Rosea from Paper Industry Waste and Production of Cellulase Enzyme

Mr. Kalpesh Vishe, Ms. Divya More, Dr. Suman K. Satyarum

Abstract :

Cellulose is polymer; main constituent in cellulosic waste contributes to environmental waste. Cellulose degrading bacteria (CDB) are organisms which selectively secretes cellulase enzyme which degrade cellulosic, lingocellulosic waste and cellulose fibers. Bioremediation principles of using microorganisms in degrading waste were used for paper industry waste to isolate CDB. Carboxy methyl cellulose agar (CMCA) was used for isolating CDB and qualitative analysis with 1% Congo red and 1M NaCl was done. Identification of Kocuria rosea was done morphologically by using Gram staining and biochemically by VITEK 2 system screening unit. Fermentation media with glucose as carbon source and submerged fermentation technique was used to extract crude cellulase enzyme. Crude cellulase activity was determined by using DNSA and estimated by Miller’s method and found to be very efficient. Different parameters like effect of pH, salt, temperature on cellulase production were also studied.