Volume : III, Issue : IX, September - 2013

Isolation and characterizations of halotolerant bacteria and identification by FAME analysis

Nidhi A. Shihora

Abstract :

Micrococcus species constitute a diverse group of bacteria widely distributed in soil and the aquatic environment. In this study, micrococcus species isolated from the coastal environment of Gujarat (India) were identified, by morphological test, physiological biochemical methods and fatty acid methyl ester (FAME) analysis, as Kocuria rosea. Growth curve of this microorganism was measured (studied) and the generation time of Kocuria rosea was 3.86 hours. The isolated culture showed salt tolerance upto 20%.It was sensitive to antibiotics like Penicillin, Streptomycin and Tetracyclin, showed decolorization of Malachite green (50mg/l) under shaking conditions within 6 hours. However, decolorization was not observed at static conditions. The decolourization was confirmed by UV –VIS spectrophotometer.  

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Nidhi A. Shihora Isolation and characterizations of halotolerant bacteria and identification by FAME analysis Indian Journal of Applied Research, Vol.III, Issue.IX September 2013

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