ISOLATION AND IDENTIFICATION OF VANCOMYCIN RESISTANCE ENTEROCOCCI FROM CLINICAL SPECIMENS

Mr.Harsha Reddy Kolli; Dr K.Swarna latha; Dr. Sreekanth basireddy; Dr Manisha Singh; Dr Vasanti Kabra

Volume : VI, Issue : VII, July - 2016

ISOLATION AND IDENTIFICATION OF VANCOMYCIN RESISTANCE ENTEROCOCCI FROM CLINICAL SPECIMENS

Mr. Harsha Reddy Kolli, Dr K. Swarna Latha, Dr. Sreekanth Basireddy, Dr Manisha Singh, Dr Vasanti Kabra

Abstract :

INTRODUCTION: Enterococci are normal inhabitants of the intestinal tract of humans. Enterococcus faecalis and E.faecium are the two most important species of human infections. vancomycin was the only drug that could be consistently relied on for the treatment of infections caused by multi drug resistance enterococci. Currently, vancomycin resistance among enterococcus isolates is a major problem worldwide. AIM OF THE STUDY: Ø To isolate and identify the enterococci from various clinical samples. Ø To detect the resistant patterns for the different species of enterococci. Ø Detecting the vancomycin resistance by disc diffusion method. Ø To determine the minimum inhibitory concentration for these resistant isolated by using the vancomycin E–strips. METHODS: A total of 2427 samples were processed during the study period out of which 108 strains of enterococci were grown in pure culture. All these enterococcal isolates were speciated and antibiotic susceptibility testing was done by Kirby Bauer method. Vancomycin resistance was determined by using the E–strips of vancomycin. RESULTS: A total of 108 Enterococci were obtained from various samples during the study period. Majority of the isolates were obtained from urine specimens. The most common species of enterococci isolated was Enterococcus faecalis accounting for 61(56.5%) followed by Enterococcus faecium 45(41.6%) and Enterococcus avium 2(1.9%). In the present study majority of the isolates were resistant to ciprofloxacin (62.03%) followed by doxycycline (49.07%). High level gentamicin resistance was seen in 44.44% of the isolates. Resistance to ampicillin and amoxyclav was almost equal accounting to 38 and 37% respectively. Resistance to vancomycin was seen in 6.5% of the total isolates and no resistance was observed for linezolid (0%). CONCLUSION: Vancomycin resistance was observed in (6.5%) isolates. Glycopeptide resistance among our isolates was high, probably reflecting the increased use of vancomycin in our hospital over the past few years. This fact highlights the importance of strict enforcement of antibiotic policies coupled with greater adherence to infection control measures to prevent emergence and spread of antibiotic resistant bacteria.