Isolation, Identification and Antimicrobial sensitivity of bacterial isolates from Pus, Sputum and Urine samples

Pradip Gamit; Mehul Chaudhari; Arvindkumar B. Dungrechiya

Volume : VI, Issue : VI, June - 2016

Isolation, Identification and Antimicrobial sensitivity of bacterial isolates from Pus, Sputum and Urine samples

Pradip Gamit, Mehul Chaudhari, Arvindkumar B. Dungrechiya

Abstract :

 A total of 8 different microbial organisms (7 bacteria and 1 Candida species) were isolated from 69 positive samples out of 155 clinical samples specimens of pus, urine, sputum and other body fluid culture collected from public health Civil Hospital Vyara.The most common isolates observed in our study were Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, S.pyrogens, Proteus ,Enterococcus spp, and Candida species. Staphylococcus aureus showed highest sensitivity towards Linezolid (92.3%). Streptococcus pyrogenes was found to be 100 % sensitive towards Vancomycin, Cefuroxzime, Cefazoline,Teicoplanin and 100% resistance towards Erythromycin,Ofloxacin, Ciprofloxacin, Cefadroxil, Gentamycin, Ampicillin + Sulbactam, Cloxacillin/ Oxacillin, Levofloxacin, Cefdinir and Linezolid. Enterococcus showed 100% sensitivity towards Ampicillin + Sulbactam and Moxifloxacin and moderate towards the rest of the antibiotics. E.coli was 90 % sensitive to most of the antibiotics. Pseudomonas Spp showed 100% sensitivity towards Ceftriaxone, Gentamycin,Piperacillin + Tazobactam. Klebsiella Spp showed highest sensitivity (89.5%) towards Ciprofloxacin.