ISOLATION, SELECTION AND FERMENTATION OF D�LIMONENE BIOTRANSFORMED MICROORGANISMS

R.SIRISHA KATHERINE; DR.M.RAVI KUMAR

Volume : VI, Issue : IX, September - 2016

ISOLATION, SELECTION AND FERMENTATION OF D�LIMONENE BIOTRANSFORMED MICROORGANISMS

R. Sirisha Katherine, Dr. M. Ravi Kumar

Abstract :

D–limonene biotransformation emerges as an attractive compared to traditional chemical methods, they proceed under mild conditions, have a prominent regio and enantio selectivity, and do not produce toxic wastes and the products obtained might be labeled as natural. The present studies involved in the isolation of microorganisms that are resistant to environment containing limonene and are able to biotransform limonene to perillyl alchol as one of reaction products. Perillyl alcohol has anticancer property against lung, liver and mammary carcinogenesis. For the isolation and screening, samples collected from specific areas of citrus processing plant (citrus fruit processing tank, machine traps, plant floor, soils at waste dumping area) and samples collected from petrol, diesel contaminated area. The samples were incubated at 300 C at 150 rpm for 48h or 7 days in YM media containing 0.1% limonene for isolation and screening purpose. Two strains were isolated from petroleum soil NPII and processing tank (ST) respectively. Thin layer chromatographic analysis of reaction products from two samples (ST, S–NPII) showed that d–limonene was biotransformed in to various reaction products may be further analyzed by techniques such as GC–MS and NMR studies to detect the products.