ß–lactamase production and antimicrobial resistances by Klebsiella species isolated from diarrheal cases among children in Kirkuk city – Iraq

Siham Sh. AL–Salihi; Ali S. Al–Jubouri; Asa‘ad F. Albayati; Yusra AR. Mahmood

Volume : VII, Issue : VII, July - 2017

ß–lactamase production and antimicrobial resistances by Klebsiella species isolated from diarrheal cases among children in Kirkuk city – Iraq

Siham Sh. Al Salihi, Ali S. Al Jubouri, Asa Ad F. Albayati, Yusra Ar. Mahmood

Abstract :

The study included the isolation and identification of 93 Klebsiella species (spp.) from 454 children suffering from acute diarrhea. The rapid standard iodometric method was used for the detection of ß–lactamase production. The Results showed that 49/93 (52.7%) Klebsiella isolates were positive for ß–lactamase production. Klebsiella spp. that showed positive result by using iodometric method, they had been tested with nitrocefin disk and spot iodometric method (filter paper method) as confirmative tests, whereby positive results were obtained by using these two methods as well. In the present study, 28/49 (57.1%) isolates appeared belonging to K. pneumoniae, 10/49 (20.4%) isolates belonging to K. oxytoca, 10/49 (20.4%) isolates belonging to K. terrigena and 1/49 (2%) isolates belonging to K. ornithinolytica.

Antibiotic sensitivity test was performed to the 49 ß–Lactamase producer using 30 different antibiotics. It was obvious that Imipenem is more active against Klebsiella spp. followed by ciprofloxacin, norfloxacin, amikacin and cefoxitin, and their resistance were (2.1%), (2.0%), (4.1%), (4.1%) and (12.2%) respectively, and all isolates showed multidrug resistance against 10 antibiotics at least. Results showed that all the isolate (100%) were able to resist ampicillin, amoxicillin, clindamycine, rifampicin, and vancomycin; while they were highly resistant to amoxicillin (97.9%), erythromycin (97.9%), carbencillin (95.9%), cephalothin (91.8%), cephradin (79.6%), cephalexin (75.5%), and pipracillin (69.4%).