Volume : V, Issue : VIII, August - 2015

Larvicidal activity of Citrullus colocynthis (L.) Schrad (Cucurbitaceae) isolated fractions against Aedes aegypti (L.),Anopheles stephensi Liston and Culex quinquefasciatus Say (Diptera: Culicidae)

Subramanian Arivoli, Rajasingh Raveen, Samuel Tennyson

Abstract :

<p><p> T Vector–borne diseases are one of the greatest contributors tohuman mortality and morbidity in tropical and subtropicalregions. Vector control remains the most effective measure and isoften the only way to prevent disease outeaks since thereare no vaccines for many vector–borne diseases.The extensive and repeated use of synthetic organic insecticides have led to disrupted natural biological control systemsheading to resurgence and resistance in insects and destruction of non–target beneficialfauna, in turn resulting in environment imbalance and human health concerns. Plants have co–evolved with insects that have equipped themwith a plethora of chemical defense, which can, in turn be usedagainst insects.In the present study, theisolated fractions of Citrullus colocynthisdichloromethanewhole plant extractwere evaluated for larvicidal activity against the vector mosquitoes viz., Aedesaegypti, Anophelesstephensi and Culexquinquefasciatus.Eight fractions viz., A, B, C, D, E, F, G and H were obtained from the residue of dichloromethane extract by column chromatography. Standard WHO protocols with minor modifications were adopted for the larvicidal bioassay. Larvicidal activity was evaluatedagainst the isolated fractions at concentrations of 25, 50, 75 and 100 ppm. Larval mortality was observed for 24 hours. Amongst the isolated fractions tested, fraction ‘C’ showed 94.4%, 96.0% and 98.4% mortality against third instar larvae of Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus at 100 ppm and LC50 values were 18.57, 23.48, 19.26 and LC90 values were 98.65, 92.26, 84.84ppm, respectively. Further investigations are needed to explore the larvicidal activity of the isolated fraction ‘C’ of dichloromethane whole plant extract of this plant and also the active ingredient(s) responsible for larvicidal activity</p></p>

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Subramanian Arivoli, Rajasingh Raveen, Samuel Tennyson Larvicidal Activityof Citrullus Colocynthis (L.) Schrad (Cucurbitaceae) Isolated Fractions Againstaedesaegypti (L.),Anophelesstephensi Liston and Culexquinquefasciatus Say (Diptera: Culicidae) Indian Journal of Applied Research, Vol.5, Issue : 8 August 2015

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