Leukocytes Count and Oxidative Stress Markers in Blood and Follicular Fluid in Infertile Women Undergo Intracytoplasmic Sperm Injection (Icsi)

Sami R. Al-Katib; Basima Sh. AlGhazali; Ban J. Edan

Volume : IV, Issue : VIII, August - 2014

Leukocytes Count and Oxidative Stress Markers in Blood and Follicular Fluid in Infertile Women Undergo Intracytoplasmic Sperm Injection (Icsi)

Sami R. Al Katib, Basima Sh. Alghazali, Ban J. Edan

Abstract :

Activated leukocytes in both of monocytes and granulocytes, generate excess reactive oxygen speciese (ROS) resulting in oxidative stress which may affect oocyte quality and ultimately affect ART success. The objectives of this study were to evaluate the association between the different leukocyte types and Malondialdehyde (MDA) levels as a free radical marker; reduced glutathione (GSH) and Catalase enzyme as antioxidant in patients undergo intracytoplasmic sperm injection (ICSI).To carry this aim a total seventy–five infertile women aged between 22–45 years(31.43 ± 5.38 years), referred to the fertility clinic in Al–Sadder teaching hospital and undergone intracytoplasmic sperm injection throughout period from March 2013 to January 2014 were included in this study. MDA, GSH and CAT were measured by spectrophotometer. The results of this study showed that there was significant positive correlation between S.MDA level and WBC count, lymphocyte count and neutrophil count.There was insignificant negative correlation between WBC count, lymphocyte count , neutrophil count and neutrophil / lymphocyte ratio with FF.GSH .While S.CAThad significant negative correlation with Eosinophil count at p<0.05.