Lower Oncogenic Potential of Human Mesenchymal Stem Cells Derived from Cord Blood Compare to Bone Marrow and Adipose Tissues

Tahereh Foroutan; Majid Sadeghizadeh

Volume : IV, Issue : IX, September - 2014

Lower Oncogenic Potential of Human Mesenchymal Stem Cells Derived from Cord Blood Compare to Bone Marrow and Adipose Tissues

Tahereh Foroutan, Majid Sadeghizadeh

Abstract :

Objective: Human mesenchymal stem cells (MSCs) derived from bone marrow (BM), cord blood (CB) and adipose (AD) tissues have potential application in cell therapy. In perspective of regenerative medicine, each of these three sources has advantages and disadvantages. The aim of the present study was comparison of reprogramming and pluripotent markers OCT4, Sox–2, c–Myc and also tumor suppressor gene P53 in MSCs derived from BM, CB and AD tissues. Methods & Materials: We analyzed the expression level of OCT4, Sox–2, c–Myc and P53 genes in hMSCs derived from BM, CB, and AD tissues by cell culture, real time RT–PCR and immunocytohcemistry. Results: Our data revealed that the expression level of pluripotent genes OCT4 and Sox–2 in MSCs derived from CB and BM were higher than AD significantly. In contrast to OCT–4A and Sox–2, the expression level of oncogenic factors c–Myc was significantly higher in MSCs from AD tissue than CB and BM. Also a significantly much lower level of P53 gene expression was detected in the MSCs from AD tissue as compared to the other sources. Our data demonstrated that human MSCs derived from CB have lower oncogenic potential compared to bone marrow and adipose tissues. Conclusion: we concluded mesenchymal stem cells derived from human cord blood have lower oncogenic potential compared to bone marrow and adipose tissues