METHYL PARATHION 50% EC INDUCED CHANGES IN ENZYMATIC ACTIVITIES OF THE FISH Channa punctatus (BLOCH)

Padmavathi; P; Veeraiah; K; Tata Rao; S; Vivek; Ch

Volume : V, Issue : I, January - 2015

METHYL PARATHION 50% EC INDUCED CHANGES IN ENZYMATIC ACTIVITIES OF THE FISH Channa punctatus (BLOCH)

Padmavathi, P, Veeraiah, K, Tata Rao, S, Vivek, Ch

Abstract :

The acute toxicity tests for 24, 48, 72 and 96 h to the fish Channa punctatus were conducted with an organophosphate pesticide methyl parathion 50% EC in both static and continuous flow&ndash;through methods. The LC50 values obtained were 0.6661, 0.6473, 0.6200 and 0.5949 ppm respectively for static, and 0.5736, 0.5592, 0.521 and 0.5364 ppm respectively for continuous flow&ndash;through method. The 1/10th of static 96 h LC50 value was taken as sub&ndash;lethal concentration and the fish were exposed to both lethal and sub&ndash;lethal concentrations for 8 days. The vital tissue of the fish viz; gill, liver, ain, muscle and kidney were isolated and analyzed for study of changes in enzymatic activity of amino&ndash;transferases, Aspartate Amino Transferase (AAT) and Alanine Amino Transferase (ALAT); Lactate dehydrogenase (LDH) and acetyl cholinesterase enzymes after 24, 96 h and 8 days of exposures. All the enzymatic activities were elevated under both lethal and sub&ndash;lethal concentrations, whereas the activity level of ACh. E activity was decreased. The changes in activity levels are more in lethal concentration compared to sub&ndash;lethal exposure. The changes in enzymatic activity are directly proportional to the increase in exposure period. These changes were in agreement with the earlier reports. The results obtained in all were discussed with the available literature.