Molecular association of Y–chromosome specific genes with semen production traits in crossbred Jersey and crossbred Holstein Friesian bulls

A. Gopinathan; S. N. Sivaselvam; S.M.K.  Karthickeyan

Volume : V, Issue : VIII, August - 2015

Molecular association of Y–chromosome specific genes with semen production traits in crossbred Jersey and crossbred Holstein Friesian bulls

A. Gopinathan, S. N. Sivaselvam, S. M. K. Karthickeyan

Abstract :

The present study was carried out to assess the polymorphism on Y–specific genes such as Sex determining region on Y– chromosome (SRY), Testis specific protein, Y– encoded(TSPY), Ubiquitin specific peptidase 9–Y linked (USP9Y) and DEAD box polypeptide 3–Y chromosome (DDX3Y) and their association with semen production traits in crossed Jersey and crossed Holstein Friesian bulls. Blood samples (5ml) collected from CBJY (80 samples) and CBHF (12 samples) bulls were used for this study. All the four Y–chromosome specific genes were highly conserved and they did not exhibit any polymorphism except SRY gene. The single nucleotide polymorphism at 641th position (G>T transversion) of SRY gene was associated with semen volume and sperm concentration in crossed Jersey bulls. The results suggested that molecular markers associated with sperm quality traits could be used in marker assisted selection of eeding bulls.