Molecular characterization of Exon–1 of Myostatin gene in Bakerwal goats in Kashmir valley

Wani A. Ahad; Sajad A. Beigh; Jaan Mohammad Wani; Rumais A Bhat; Mudasir Andrabi; Masroor Hussain; Hina F. Bhat; Riaz A. Shah

Volume : VII, Issue : VIII, August - 2017

Molecular characterization of Exon–1 of Myostatin gene in Bakerwal goats in Kashmir valley

Wani A. Ahad, Sajad A. Beigh, Jaan Mohammad Wani, Rumais A Bhat, Mudasir Andrabi, Masroor Hussain, Hina F. Bhat, Riaz A. Shah

Abstract :

Myostatin or growth differentiation factor 8 (GDF8), is a skeletal muscle regulator factor that determines muscle mass in animals including human beings. Myostatin, a protein has a remarkable role in growth and muscular development. Mutations in myostatin gene leads to the double muscling phenomenon in my animals. The present investigation was aimed at studying the genetic variation and characterization of exon–1 of myostatin gene in Bakerwal goats in Kashmir valley by PCR–DNA Sequencing method. And a 492–bp fragment of the exon–1 of MSTN gene was amplified by PCR using oligonucleotide primers standardized for Ovis aries species. The sizes of the amplification products were similar in sheep and goat. Myostatin gene in bakerwal goats reveals monomorphism since no variation was found. The result indicates strong conservation of DNA sequence between sheep and bakerwal goats.