Volume : VII, Issue : VIII, August - 2017

Molecular characterization of fish pathogens & factors influencing pathogenicity

Prarthana, J, Maruthi, K, R

Abstract :

 Fish farming, is an fast growing business in aquaculture relies on mass culturing of fishes for their increasing demand on fish products, but  forced confinement of farmed fish may in some circumstances make them more prone to be affected by environmental stressors  leading to infectious disease causing heavy losses to fish farmers. Environmental stressors include adverse chemical condition of the water. Fish become susceptible to bacterial pathogens during stressed condition due to changes in ammonia ,potassium concentration ,pH & temperature fluctuation, created an unbalanced host, pathogen & water environment .The present study is on diseased fresh water fish , Tilapia , interplay of physiological factors contributing  towards pathogenicity of bacteria responsible for infection. Infected fish was identified with hemorrhagic skin near gills ,skin peel off ,pop out eye, slight discoloration. From various parts of the infected fish the biopsy were taken. Skin scraping, gills, intestine, kidney, serially diluted & inoculated to nutrient to nutrient oth & stained , all the bacterial isolates are grown on chemically defined media to check for their nutritional requirement ,further to determine the pathogenicity of bacterial isolates , growth is analyzed ai different concentration of ammonia ,potassium with pH & temperature gradient .As a standard , auxenic cultures of Aeromonas hydrophila is studied .Finally molecular characterization of two unknown isolates studies for various physiological parameter were identified to be as Flavobacterium Columnare & Streptococcus  agalacitace.

Keywords :

Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Prarthana, J, Maruthi,K,R, Molecular characterization of fish pathogens & factors influencing pathogenicity, INDIAN JOURNAL OF APPLIED RESEARCH : Volume‾7 | Issue‾8 | August‾2017

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