Volume : IV, Issue : XII, December - 2014

Molecular Cloning of a Novel Putative Retrotransposon From Aloe vera

Sharma A Suvarna, Kadirvelu Sharon, Harshal Anupma

Abstract :

<p>Aloe vera is a member of the family Liliceae and has medicinal properties. It is a sturdy plant that withstands abiotic stresses such as extreme temperature and drought conditions. It is possible that Aloe< /> maybe harboring stress inducible genes in its genome. HVA22 is a highly inducible gene for Abscisic acid and environmental stress in Barley. We followed the PCR approach and used Aloe genomic DNA as template along with primers specific against YOP1 (homolog of HVA22 in Yeast) for amplification. Amplicon obtained was 545bp; sequencing< /> and bioinformatics analysis revealed it to be a gag_ protease region of a Ty3– gypsy retrotransposon. Aloe cDNA pool< /> was used to obtain a transcript of the same gene. Plant retrotransposons are linked with abiotic and biotic stresses< /> with respect to its effect on retrotransposon’s activation and function. In this study, transcript of gag_protease region< /> of a Ty3 retrotransposon was isolated from Aloe vera. </p>

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Sharma A Suvarna,Kadirvelu Sharon,Harshal Anupma Molecular Cloning of a Novel Putative Retrotransposon From Aloe Vera Indian Journal of Applied Research, Vol.4, Issue : 12 December 2014

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