Volume : VI, Issue : VIII, August - 2016

Molecular Line Probe Assay for the detection of multi drug resistant tuberculosis and comparison of results with conventional solid culture and drug susceptibility testing

Manoj Kumar Dubey, Uma Bhardwaj, M. Hanif, Kamal Kishore Chopra, Aswani Khanna, Kaushal Kumar Dwivedi, Himanshu Vashishtha, Sanjeev Saini, Zeeshan Sidiq, Vasim Ahmad, Srashty Sharma

Abstract :

 Background: Drug–resistant TB often goes undetected and untreated in many countries. The laboratory is an essential component in TB control programs to provide culture and drug susceptibility test services. In this study we evaluated the performance of line probe assay (LPA) in comparison with LJ culture DST. Methods: Samples from Three thousand and sixty two (N=3062) MDR–TB suspects were included in the study. Sputum smear microscopy was done for all the specimens and all smear positive specimens were processed for LPA and LJ culture and DST. Results: Overall concordance between conventional DST and LPA was observed among 652 specimens. In comparison to 238 culture isolates observed sensitive on LJ C–DST, 226 were found sensitive by LPA (concordance=95%). Further, In comparison to 426 culture isolates observed resistant on LJ C–DST, 12 more cases were found resistant by LPA (Concordance= 97.3%).Conclusion: Overall concordance of the LPA with conventional DST was 96%

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Manoj Kumar Dubey, Uma Bhardwaj, M.Hanif, Kamal Kishore Chopra, Aswani Khanna, Kaushal Kumar Dwivedi, Himanshu Vashishtha, Sanjeev Saini, Zeeshan Sidiq, Vasim Ahmad, Srashty Sharma Molecular Line Probe Assay for the detection of multi drug resistant tuberculosis and comparison of results with conventional solid culture and drug susceptibility testing Indian Journal of Applied Research,Volume : 6 | Issue : 8 | August 2016

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