Volume : V, Issue : V, May - 2015

Molecular Study of mitochondrial cytochrome oxidase subunit 1 gene in the oocyte in vitro maturation in the Iraqi ewes

Hayder Abdul Kareem Al Mutar, Saad Akram Hatif, Najwa Sh. Ahmed

Abstract :

 This study was conducted on 668 ovary from abattoir of adult ewes. Three types oocytes aspirated from  mature follicles (Oocyte with cumulus cells , partial surrounded by cumulus cells ,and oocytes without cumulus cells ) it is incubated in the Co 2 incubator to maturation in TCM199 .The step of oocyte maturation previously  the in vitro fertilization, therefore the investigation of oocyte in the molecular level very important for detection the  oocyte quality ,so the defect in the mitochondrial gene Cytochrome Oxidase subunit 1 (CO1) may take place through  the oocyte cultured in the tissue culture media. Subsequent by genomic DNA extraction by (Bioneer Kit), to investigation the goal gene, samples yielded evidence of amplified CO1 gene on the agarose gel electrophoresis. No mutation in the CO1 sequence compared with the Al–naemi sheep eed and Assaf sheep eed . The conclusion , via PCR  and sequencing of CO1 gene, no differences in the oocytes through the oocyte in vitro maturation in the age 3–6  old of ewes.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Hayder Abdul-Kareem AL-Mutar, Saad Akram Hatif, Najwa Sh. Ahmed Molecular Study of mitochondrial cytochrome oxidase subunit 1 gene in the oocyte in vitro maturation in the Iraqi ewes Indian Journal of Applied Research, Vol.5, Issue : 5 May 2015

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