Multiplex PCR for detection of virulence gene profiles of Salmonella isolates from animals and man

Arundhati Purkayastha; Probodh Borah; Rajiv K. Sharma; Partha Pratim Borah; Mridusmita Choudhury

Volume : V, Issue : XII, December - 2015

Multiplex PCR for detection of virulence gene profiles of Salmonella isolates from animals and man

Arundhati Purkayastha, Probodh Borah, Rajiv K. Sharma, Partha Pratim Borah, Mridusmita Choudhury

Abstract :

Salmonellosis is a leading cause of illness in animals and man worldwide. The present study was undertaken to isolate Salmonella from various sources including animals, birds and human and to study their virulence gene profiles based on multiplex PCR assays developed for the purpose. Out of a total of 453 samples examined, 30 (6.62%) were found to be positive for Salmonella. Among the 30 isolates recovered, the serovar Salmonella Enteritidis was found to be the most prevalent (53.33%). The other serovars recovered were Salmonella Typhi 6 (20.00%), Salmonella Typhimurium 4 (13.33%), Salmonella Weltevreden 2 (6.67%), and Salmonella Dublin and Salmonella Gallinarum 1 (3.33%) each. A multiplex PCR was designed for rapid detection of six important virulence genes of Salmonella, the chromosomal genes invA, agfA and stn in one set, and the plasmid encoded genes spvA, spv B and spv C in another. Isolates belonging to different serovars showed variable results in respect to possession of different virulence genes.