Volume : VII, Issue : VII, July - 2017

Occurrence of Broad Spectrum Antibacterial Agents in Roots of Paeonia officinalis L.

Murtaza Abid, Abbas Ali Mahdi

Abstract :

 Folkloric medicinal application of Paeonia officinalis L. (Family: Paeoniaceae) in the inhibition of bacteria was investigated. Phytochemical analysis revealed the presence of carbohydrates, glycosides, steroids, triterpenes, alkaloids,tannins and saponins. Hexane (HE), dichloromethane (DCM), ethyl acetate (EA) and methanol (ME) extracts of P. officinalis L. roots were evaluated for antibacterial activity, against pathogenic bacteria, Shigella dysenteriae, Salmonella typhi, Corynebacterium ulcerans, Klebsiella pneumonia, Staphylococcus aureus, Methicillin resistant Staphillococus aureus (MRSA), Streptococcus pyogens, Baccillus cereus, Escherichia coli and Enterobacter sp, using the agar–in–well diffusion method. Determination of zone of inhibition (ZI) showed inhibition ranging from 20–23 mm (HE), 25–30 mm (DCM), 30–33 mm (EA) and 22–25 mm (ME) against the entire test organisms except Methicillin resistant Staphillococus aureus (MRSA), Klebsiella pneumonia and Shigella dysenteriae. The results of the minimum inhibitory concentration (MIC) showed that EA fraction inhibited the growth of all test organisms at a low concentration of 5 mg/ml. Higher MIC values were observed for DCM (5–10 mg/ml), HE and ME fraction all showed MIC at 10 mg/ml. The microorganisms were completely killed at a higher concentration; EA (MBC/MFC; 10 mg/ml), DCM (MBC/MFC; 10–20 mg/ml), ME and HE (MBC/MFC; 20 mg/ml). Bacterial evaluation reveals that the HE extract had the highest activity with MIC of 0.675 mg/ml, followed by DCM extract. The results clearly showed that the plant had potential that can be explored in the search for anti–bacterial drug.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Murtaza Abid, Abbas Ali Mahdi, Occurrence of Broad Spectrum Antibacterial Agents in Roots of Paeonia officinalis L., INDIAN JOURNAL OF APPLIED RESEARCH : Volume‾7 | Issue‾7 | July‾2017

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