Occurrence of Esbl and Mbl in Clinical Isolates of  Pseudomonas Aeruginosa � An Emerging Threat to  Clinical Therapeutics

Velvizhi G; Sucilathangam G; Anna T

Volume : III, Issue : IX, September - 2013

Occurrence of Esbl and Mbl in Clinical Isolates of Pseudomonas Aeruginosa � An Emerging Threat to Clinical Therapeutics

Velvizhi G, Sucilathangam G, Anna T

Abstract :

The present study was undertaken to detect the Extended Spectrum β Lactamases (ESBL) and Metallo β Lactamases (MBL) in isolates of Pseudomonas aeruginosa which were isolated from wound infections and to evaluate their susceptibility patterns. The presence of the ESBL enzyme was detected by the phenotypic confirmatory test and the MBL enzyme was detected by the Imipenem – EDTA Double Disk Synergy test and Modified Hodge test. Out of 100 samples which were cultured, 26[26 %] yielded the growth of Pseudomonas aeruginosa.Among the 26 Pseudomonas aeruginosa isolates, 8 [30.76%] were ESBL producers and 4[15.4%] isolates were metallo β lactamase producers. The modified Hodge test detected 2 strains as metallo–β–lactamase producers (MBL).The EDTA disc synergy test detected the same 2 strains as well as an additional two strains as MBL producers.All the ESBL producing isolates were sensitive to Imipenem, while the MBL producing isolates showed widespread resistance to Aminoglycosides, Ciprofloxacin and the Piperacillin with Tazobactam combination. The present study underlines the unique problem that the presence of ESBL and MBL mediated resistance in Pseudomonas aeruginosa has created a therapeutic challenge for the clinicians and microbiologists. Hence, we suggest that the detection of ESBL and MBL in Pseudomonas aeruginosa should be a routine practice in the hospital.