Volume : V, Issue : V, May - 2015

OPTIMIZATION AND CHARACTERIZATION OF LIPOPEPTIDE BIOSURFACTANT

Dr. Priya Iyer, P. Sandhya

Abstract :

Lipopeptides are the bioactive peptides and some constituents of these compounds are surfactin, fengycin, iturins, mycosubtilins and bacillomycins. Among these lipopeptides, surfactin is produced by Bacillus  subtilis that has strong anti–microbial,anti–viral and anti–larvicidal activities. In the present study, Bacillus spp. was chosen for lipopeptide production and the growth medium used was whey + mineral salt media. The optical density of the  growth medium was checked for 72 and 96 hours and the best results were observed in 72 hour culture; thus chosen  for lipopeptide production.The biomass and the crude obtained were estimated to be 21.72 g/L and 5.0 g/L respectively. The crude obtained was characterized for its surfactant molecule using MALDI–MS and FTIR. MALDI–MS showed  the highest peak of 1029.8 at the intensity of 69.4 indicating the presence of surfactin molecule. FTIR also proved the  presence of surfactin 

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Dr.Priya Iyer, P.Sandhya OPTIMIZATION AND CHARACTERIZATION OF LIPOPEPTIDE BIOSURFACTANT Indian Journal of Applied Research, Vol.5, Issue : 5 May 2015

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