Optimization of Physico–Chemical Parameters for the Extraction of Catechins from seeds of Hibiscus sabdariffa species.

H.Padmaja; Meena Vangalapati

Volume : IV, Issue : XII, December - 2014

Optimization of Physico–Chemical Parameters for the Extraction of Catechins from seeds of Hibiscus sabdariffa species.

H. Padmaja, Meena Vangalapati

Abstract :

Hibiscus sabdariffa L, an annual herbaceous shrub commonly called as Roselle has many industrial, pharmaceutical uses in many countries all over the world. Phytochemical composition of Hibiscus sabdariffa includes alkaloids, anthocyanins, flavonoids, polyphenols, quinones, saponins, tannins, terpenes and sterols. The objectives of this work were extraction of group of phenolic compounds called catechins (Flavanols) from the seeds of Hibiscus sabdariffa. The present studies target on optimization of physico–chemical parameters like effects of different solvents, different solvent percentages, pH, particle size of the seed powder and the soaking time for the extraction of catechins from the seeds of Roselle. For the extraction of Catechins, the optimum results were observed for the effects of different solvents, different solvent percentages, pH, particle size of the seed powder and the soaking time as ethanol, 100%(v/v), pH 4.0, microns, and 2days respectively. The highest Catechin concentration for optimized conditions was 485mg/l.