ORANGE PEEL WASTE FOR AMYLASE PRODUCTION FROM Bacillus sp. USING RESPONSE SURFACE METHODOLOGY

Kalaivani. S.; Suganya. A.; Shyamala. S. Y.; Gopinath; L. R; Archaya. S.

Volume : V, Issue : VIII, August - 2015

ORANGE PEEL WASTE FOR AMYLASE PRODUCTION FROM Bacillus sp. USING RESPONSE SURFACE METHODOLOGY

Kalaivani. S. , Suganya. A. , Shyamala. S. Y. , Gopinath, L. R, Archaya. S.

Abstract :

Amylase production using orange peel as substrate by Bacillus subtilis. Bacillus sp. were isolated from soil contaminated with decaying material of fruit waste. Bacillus sp. EB–9 showed maximum halo zone on starch agar plate (27mm) which was considered as promising isolate for amylase production The nutritional components and operating conditions such as orange peel, glucose, yeast extract, MgSO4, KH2PO4, K2HPO4, NaCl, inoculum size and incubation period were varied according to the Plackett–Burman statistical designs. The variation of α– amylase production in the fifteen trials of Box–Behnken design was ranged from 102.27 to286.62 U /min /ml. The optimum value of the variables in actual unit was predicted as orange peel (4.27 %), yeast extract (0.44 %) and incubation period (43.39 h), with the predicted maximum α– amylase production of 287.92 U/min/ml of fermented media. A mean value of 290.42 ± 0.2462 U/min/ml of α– amylase was acquired from real experiments.