Oxidative stress & antioxidants status in patients with different stages of Oral squamous cell carcinoma  In–vitro treatment with Neem Leaf Extract(NLE)

Abhishek Sharma; Nidhi Sharma; Najmul Islam

Volume : VI, Issue : III, March - 2016

Oxidative stress & antioxidants status in patients with different stages of Oral squamous cell carcinoma In–vitro treatment with Neem Leaf Extract(NLE)

Abhishek Sharma, Nidhi Sharma, Najmul Islam

Abstract :

<p>&nbsp;Objective: to study level of oxidative stress and antioxidant status in oral cancer patients with various clinical stages before and after in&ndash;vitro treatment with Neam Leaf Extract(NLE) in different concentrations. Methods: Blood samples of 60 oral cancer patients with various clinical stages of oral cancer and 20 age and sex matched healthy subjects were collected. Plasma and erythrocytes levels of TBARS, GSH, and activities of SOD, CAT and GPx and ferric reducing ability of plasma ,were assayed using specific colorimetric methods. The statistical comparisons were performed by ANOVA followed by Student&rsquo;s t&ndash;test. Results: The TBARS levels were gradually increased whereas antioxidants were gradually reduced from stage II to stage IV of oral cancer patients and in comparative study done after treating the whole blood with different concentrations of NLE, it was found that oxidative stress was ameliorated and antioxidant status was increased . conclusion: The altered lipid peroxidation in plasma and erythrocytes of oral cancer patients may be related to their compensatory changes in the antioxidants defense system.</p>