Volume : III, Issue : VII, July - 2013

Partial purification and Characterization of enterocins produced by E. faecalis and E. faecium

Nemade S. P. , Musaddiq M.

Abstract :

Bacteriocins produced by lactic acid bacteria have received increasing attention due to their potential use as natural preservatives and as natural substitute for chemical preservatives. In this study, a bacteriocin, produced by Enterococcus faecalis and Enterococcus faecium referred to as enterocin, was partially purified and characterzed. Results showed that enterocins was heat stable and stable over a wide pH range. The proteinaceous nature of the antimicrobial compound was ascertained by its sensitivity to many proteolytic enzymes confirming it to be a bacteriocin. Enterocin was partially purified by ammonium sulphate precipitation technique, and the analysis by 16% tris– tricin sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS–PAGE), showed a molecular mass of Enterocins was estimated to be 4.3 to 5.3 kDa.  

Keywords :

Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Nemade S.P., Musaddiq M. Partial purification and Characterization of enterocins produced by E. faecalis and E. faecium Indian Journal of Applied Research, Vol.III, Issue.VII July 2013

Number of Downloads : 695

References :