Volume : X, Issue : VII, July - 2020

PCR-RFLP assay identification of Non-albicans Candida species by MspI restriction enzyme.

Gunjan Dutta, Dr Anita Pandey

Abstract :

Introduction– Non albicans Candida (NAC) are most commonly isolated from various clinical specimens. The conventional methods use for the identification are time consuming and difficult to perform. Aim– To identify NAC species from various clinical specimens using polymerase chain reaction (PCR) and Restriction fragment length polymorphism (RFLP) with MspI restriction enzyme. Materials and methods – By using universal primers, ITS1 and ITS4, we could amplify ITS1 and ITS2 rDNA region in 79 NAC isolates. Result– in our study we successfully identified all isolates using MspI restriction enzyme separately. C.tropicalis was identified as the most common species (74.68%) followed by C.parapsilosis, C.glaata, C.krusei and C.guilliermondii. Conclusion– PCR–RFLP method by using ITS1 and ITS4 primers and the restriction enzyme is rapid and reliable method in clinical laboratories for identification of medically important NAC species.

Keywords :

Article: Download PDF    DOI : 10.36106/ijar  

Cite This Article:

PCR-RFLP ASSAY IDENTIFICATION OF NON-ALBICANS CANDIDA SPECIES BY MSPI RESTRICTION ENZYME., Gunjan Dutta, Dr Anita Pandey INDIAN JOURNAL OF APPLIED RESEARCH : Volume-10 | Issue-7 | July-2020

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