PHENOTYPIC CHARACTERIZATION AND ANTIFUNGAL SUSCEPTIBILITY PATTERN OF CANDIDA SPECIES IN TERTIARY CARE CENTER

Meena K; Gandhi S; Jain B S; Ranjan N

Volume : VIII, Issue : IV, April - 2018

PHENOTYPIC CHARACTERIZATION AND ANTIFUNGAL SUSCEPTIBILITY PATTERN OF CANDIDA SPECIES IN TERTIARY CARE CENTER

Meena K, Gandhi S, Jain B S, Ranjan N

Abstract :

The number of non albicansCandida species isolated from clinical samples is increasing continuously every year. The present study is prospective, which aimed to phenotypically characterize the candida species and their antifungal susceptibility pattern. All samples were subjected to KOH wet mount, gram’s staining and culture on Sabouraud’s dextrose agar. Further speciation was done by germ tube test, corn meal agar inoculation and carbohydrate utilization. Susceptibility testing was done by disc diffusion method (fluconazole, itraconazole and ketoconazole disc). Among total 317 Candida isolates, non albicansC. tropicalis(57) was predominant isolate. Resistance to fluconazole was maximum 59.93% and resistance to ketoconazole was 40.7%.

Summary: In present study non albicansCandida species showed relatively higher resistance to itraconazole. Species–level identification of Candida and their antifungal sensitivity testing should be performed to achieve better clinical result.