Volume : IV, Issue : I, January - 2014

Pregnancy diagnosis by seed germination inhibition test in mithun (Bos frontalis) cows

P. Perumal,

Abstract :

The present study was undertaken to assess the seed germination inhibition test on pregnancy diagnosis in mithun cows. Total of 14 mithun were selected to the study and divided into positive group (pregnant cows; n=7) and negative group (non- pregnant; n=7). The urine was collected through manual method from the pregnant and non- pregnant mithun cows. The urine from the both groups was diluted with distilled water at the ratio of 1:4. Fifteen good quality germinatable paddy seed were taken in sterile petri dishes and 15 ml of diluted urine was added. For each cow the test was conducted with a replica of six tests in six petri dishes. Paddy seeds with water only were also carried out as the control group. Seed germination inhibition percentage and shoot length were measured from three groups after three days. The result revealed that there was significant difference between the three groups of mithun cows in seed germination inhibition percentage and shoot length. It was concluded that the seed germination inhibition technique is useful to detect pregnancy in mithun cows as a simple, non- invasive and economical method. Future, estimation of hormones such as progesterone, estrogen and correlate with seed germination inhibition percentage and shoot length at different trimester of pregnancy is warranted to confirm the present findings.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

P. Perumal, Pregnancy diagnosis by seed germination inhibition test in mithun (Bos frontalis) cows Indian Journal of Applied Research, Vol.IV, Issue. I

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