Prevalence of Methicillin resistance in staphylococci in a tertiary care hospital.

Arpandeep kaur; Dr Bimla Devi; Dr Kanwardeep Singh; Dr Pushpa Devi; Dr Shailpreet Kaur

Volume : V, Issue : XI, November - 2015

Prevalence of Methicillin resistance in staphylococci in a tertiary care hospital.

Arpandeep Kaur, Dr Bimla Devi, Dr Kanwardeep Singh, Dr Pushpa Devi, Dr Shailpreet Kaur

Abstract :

 Methicillin resistant Staphylococcus aureus (MRSA) and methicillin resistant coagulase negative staphylococcus (MRCONS) are associated with high morbidity and mortality rates. The present study was undertaken to know their prevalence in a tertiary care hospital of Amritsar, Punjab. A total of 6429 pus and urine samples received from indoor and outdoor patients from November 2013 to June 2014 were processed as per standard procedures. Out of the total 6429 samples 306 (15.75 %) staphylococcus were isolated. These samples were identified by gram stain, coagulase, catalase and other biochemical reactions. Antimicrobial susceptibility testing was done by Kirby –Bauer disc diffusion test and Methicillin resistance was tested using cefoxitin 30 microgram disc on Muller Hinton Agar. Out of 306 staphylococci isolates 216 (70.4%) were S.aureus and 90 (29.6%) were coagulase negative staphylococci (CONS). Methicillin resistance was found in 112 (51.8%) of S.aureus and 47(52.2%) in CONS by cefoxitin disc diffusion method.