Volume : V, Issue : IV, April - 2015

Prevalence of multidrug resistant altered Vibrio cholerae O1 isolates among Diarrhoeal patients in Delhi during 2008–2012.

Puneeta Singh, Dhirendra Kumar, Yogendra Prasad, Thandavarayan Ramamurthy, Banwari Lal Sarkar, Naresh Chand Sharma

Abstract :

 Altered Viio cholerae O1 has been reported from some areas in the country as well as from the other 

countries. Delhi is an endemic area for toxigenic V. cholerae O1. Therefore, we were interested to evaluate the presence of altered Viios, their drug resistance status and phage types among the strains isolated from 
suspected cholera/acute gastroenteritis patients admitted in this hospital. A total of 59 isolates representing different 
years were used for D –MAMA PCR to detect the Haitian genotype (ctxB7) strains and classical genotype (ctxB1). A 
subset of 49 isolates was tested to determine the phage types. All selected strains were serologically positive for V. 
cholerae O1, serotype Ogawa and biochemically biotype El Tor. The antibiotic susceptibility test explicated that 100% 
strains were resistant to cotrimoxazole and nalidixic acid. Phage typing revealed that 79.6% of isolates were phage 
type T–27. D–MAMA PCR, has shown 52.5% strains were carrying ctxB7 gene since 2008. 

Keywords :

Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Puneeta Singh, Dhirendra Kumar, Yogendra Prasad, Thandavarayan Ramamurthy, Banwari Lal Sarkar, Naresh Chand Sharma Prevalence of multidrug resistant altered Vibrio cholerae O1 isolates among Diarrhoeal patients in Delhi during 2008–2012. Indian Journal of Applied Research, Vol.5, Issue : 4 April 2015


Number of Downloads : 824


References :