Volume : III, Issue : IX, September - 2013

Production of Amylase Enzyme Using Aspergillus niger

Vishal Pawar

Abstract :

Amylase is an enzyme produced by different fungi and bacteria. For the production of the amylase enzyme the fungus was isolated form soil by dilution method. The medium used for the isolation was Czapek–Dox medium. From onion roots the same fungus was isolated and characterized. The fungus characterized on the Potato Dextrose Agar medium containing 1% starch. The Aspergillus niger colonies showed maxium zone of clearance after pouring iodine were picked up and used. In this fermentation for the production of the amylase enzyme, Aspergilus niger isolated from soil as well as onion roots used. The production was carried out using 5lit fermenter on lab scale. The media for the fermentation is optimized in the laboratory and used for the isolated Aspergillus niger. The product produced using fermentation for 72hrs at 30oC. The produced product was separated by filtration, centrifugation and salt precipitation method.  

Keywords :

Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Vishal Pawar Production of Amylase Enzyme Using Aspergillus niger Indian Journal of Applied Research, Vol.III, Issue.IX September 2013

Number of Downloads : 839

References :