Volume : III, Issue : III, March - 2013

Production of Amylase from Marine Fungus Using Spoiled Banana Fruit as a Substrate

R. Sathya, T. Ushadevi

Abstract :

Solid state fermentation was carried out using banana powder as a substrate for the production of amylase through marine fungus. Totally twenty different kinds of fungal species were isolated from marine sediments, and screened for amylase enzyme production. Among them two species, such as Aspergillus oryzae and Penicillium sp. were selected based on their high level of amylase activity. Banana (fruit) powder was used as a substrate for high yield of amylase production during very short period. Mineral salt medium with different carbon sources (sucrose, glucose, starch) were used for the optimization of enzyme production. The starch supplementation showed high level of enzyme activity than glucose and sucrose. The growth and amylase production of A. oryzae and Penicillium sp on banana fruit substrate with different carbon sources, incubation time, pH and temperature were studied. The optimum conditions for amylase production were determined. The enzyme activity was marginally better in Aspergillus oryzae than Penicillium sp.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

R. Sathya,T. Ushadevi Production of Amylase from Marine Fungus Using Spoiled Banana Fruit as a Substrate Indian Journal of Applied Research, Vol.III, Issue.III March 2013

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