Production of Antibiotics Isolated from Soil Bacteria from Rhizospheric and Non–Rhizospheric Region of Medicinal Plants

Amit Pandey; Tanya Malviya

Volume : IV, Issue : VIII, August - 2014

Production of Antibiotics Isolated from Soil Bacteria from Rhizospheric and Non–Rhizospheric Region of Medicinal Plants

Amit Pandey, Tanya Malviya

Abstract :

The present study was carried out to explore the production of antibiotics from soil microbes of medicinal plants. These microbes were isolated from the rhizospheric and non–rhizospheric region of two medicinal plants–Oscimum tenuiflorum and Aloe barbadensis. The bacterial cultures present in these soil samples were isolated by primary screening and sub–culturing methods. The results of Antibiotic Sensitivity Test revealed the activity of isolates which further led to characterization of isolates through Gram’s staining and Bergey’s manual. The isolates obtained were Proteus vulgaris, Streptococcus epidermis, Lactobacillus fermentum, Bacillus cereus, N.mucosa, Sterptococcus equisimilis, Streptococcus faecalis and Bacillus subtilis. These isolates were cultured in optimized production media in suitable nutrient sources, temperature conditions and pH range. Glucose and sucrose as carbon sources and ammonium chloride and ammonium sulphate as nitrogen sources at 370C and pH–7 were suitable for growth of majority isolates. This further led to secondary screening by intracellular and extracellular metabolite extraction from the cultures obtained in production media. These antimicrobial properties of the metabolites were tested by AST which gave positive results. This led to determination of antimicrobial compounds or antibiotics in secondary metabolites of cultures by chromatographic techniques. The results i.e. Rf values obtained from the silica gel Thin Layer Chromatography proved the possible presence of beta–lactum antibiotics in the secondary metabolites extracted from the cultures of Lactobacillus fermentum, Bacillus cereus and N.mucosa.